3KKB
The crystal structure of a two-component sensor domain from Pseudomonas aeruginosa PA01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97942 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 75.361, 75.361, 75.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.680 - 1.881 |
R-factor | 0.1662 |
Rwork | 0.163 |
R-free | 0.22010 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.876 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 1.910 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.094 | 0.541 |
Number of reflections | 20202 | |
<I/σ(I)> | 23.8 | 2.3 |
Completeness [%] | 98.8 | 86.3 |
Redundancy | 5.4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 0.1M Phosphate-citrate, 1.6M NaH2PO4 /0.4M K2HPO4, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |