3KJZ

Crystal structure of native peptidyl-tRNA hydrolase from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	2009-03-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	45.010, 58.980, 62.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.430 - 2.400
R-factor	0.182
Rwork	0.182
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z2i
RMSD bond length	0.008
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.430	2.500
High resolution limit [Å]	2.400	2.400
Number of reflections	6501
Completeness [%]	95.0	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20mM Tris-HCl, 1mM EDTA, 50mM NaCl, 10% isopropanol, 30% PEG 1500, 5mM 2-mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K