3KJX
Crystal structure of a transcriptional regulator, Lacl family protein from Silicibacter pomeroyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.662, 137.392, 154.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.290 - 2.330 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.290 | 2.420 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.109 | 0.628 |
| Number of reflections | 70020 | |
| <I/σ(I)> | 10 | 2.5 |
| Completeness [%] | 98.8 | 97.6 |
| Redundancy | 11.5 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M Lithium sulfate monohydrate, 0.1M Tris-Hcl, 30% PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
