3KJX
Crystal structure of a transcriptional regulator, Lacl family protein from Silicibacter pomeroyi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.662, 137.392, 154.984 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.290 - 2.330 |
R-factor | 0.238 |
Rwork | 0.238 |
R-free | 0.28700 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.290 | 2.420 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.109 | 0.628 |
Number of reflections | 70020 | |
<I/σ(I)> | 10 | 2.5 |
Completeness [%] | 98.8 | 97.6 |
Redundancy | 11.5 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M Lithium sulfate monohydrate, 0.1M Tris-Hcl, 30% PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |