3KJV
HIV-1 reverse transcriptase in complex with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 165.423, 171.973, 103.398 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.840 - 3.100 |
R-factor | 0.243 |
Rwork | 0.239 |
R-free | 0.32100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hmi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | CNX (2005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.104 | 0.537 |
Number of reflections | 25754 | |
<I/σ(I)> | 9.27 | 1.8 |
Completeness [%] | 94.4 | 95.4 |
Redundancy | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 3% PEG 4000, 0.05M MES pH 6.0, 5mM magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |