3KJT
Stimulation of the maltose transporter by a mutant sucrose binding protein gives insights into ABC transporter coupling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-03-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.890, 65.540, 57.500 |
| Unit cell angles | 90.00, 101.14, 90.00 |
Refinement procedure
| Resolution | 24.800 - 1.500 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.800 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.048 | 0.329 |
| Number of reflections | 48552 | |
| <I/σ(I)> | 17.79 | 2.03 |
| Completeness [%] | 85.4 | 82.4 |
| Redundancy | 3.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | PEG MME 5000, 0.1M Sodium acetate, 60mM MgCl2, 10mM ZnCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
