3KJI
Zn and ADP bound state of CooC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-30 |
| Detector | MX-225 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.353, 81.760, 86.578 |
| Unit cell angles | 90.00, 128.88, 90.00 |
Refinement procedure
| Resolution | 19.690 - 2.130 |
| Rwork | 0.224 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.489 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.190 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 34673 | |
| Completeness [%] | 99.1 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 290 | 0.2 M Lithium citrate tribasic, 10% PEG3350, 5% Glycerol, 10 mM AlF4, 10 mM MgCl2, 1 mM ADP, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
