3KIH
The crystal structures of two fragments truncated from 5-bladed beta-propeller lectin, tachylectin-2 (Lib2-D2-15)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-04-06 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.566, 56.418, 86.306 |
Unit cell angles | 90.00, 122.90, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.490 |
R-factor | 0.23654 |
Rwork | 0.233 |
R-free | 0.29903 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tl2 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.520 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.490 | 2.500 |
Number of reflections | 17718 | |
Completeness [%] | 98.9 | 90.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil | 7 | 298 | 100mM HEPES, 0.2M magnesium chloride, 20% PEG 6000, pH 7, Microbatch under oil, temperature 298K |