3KIH
The crystal structures of two fragments truncated from 5-bladed beta-propeller lectin, tachylectin-2 (Lib2-D2-15)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.566, 56.418, 86.306 |
| Unit cell angles | 90.00, 122.90, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.490 |
| R-factor | 0.23654 |
| Rwork | 0.233 |
| R-free | 0.29903 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tl2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.520 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.490 | 2.500 |
| Number of reflections | 17718 | |
| Completeness [%] | 98.9 | 90.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 7 | 298 | 100mM HEPES, 0.2M magnesium chloride, 20% PEG 6000, pH 7, Microbatch under oil, temperature 298K |
