3KIF
The crystal structures of two fragments truncated from 5-bladed beta-propeller lectin, tachylectin-2 (Lib1-B7-18 and Lib2-D2-15)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 |
| Unit cell lengths | 80.558, 80.558, 170.681 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.21625 |
| Rwork | 0.213 |
| R-free | 0.27613 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.420 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.544 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.118 | 0.452 |
| Number of reflections | 42804 | |
| <I/σ(I)> | 34.6 | 4.2 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 6.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | 100mM Bis-Tris, 0.2M lithium chloride, 19% PEG 3350, pH 5.5, VAPOR DIFFUSION, temperature 298K |
