3KHY
Crystal Structure of a propionate kinase from Francisella tularensis subsp. tularensis SCHU S4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-23 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.079, 87.127, 149.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.510 - 1.978 |
| R-factor | 0.1553 |
| Rwork | 0.155 |
| R-free | 0.19330 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.4_115) |
| Refinement software | PHENIX ((phenix.refine: 1.4_115)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.124 | 0.490 |
| Number of reflections | 117233 | |
| <I/σ(I)> | 18 | 5.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | NaFormate 4M, 0.3M 195 NDSB, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
