3KHT
Crystal structure of response regulator from Hahella chejuensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-22 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 57.090, 57.090, 200.456 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.750 - 2.100 |
| R-factor | 0.227 |
| Rwork | 0.224 |
| R-free | 0.28800 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.965 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.750 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.074 | 0.403 |
| Number of reflections | 11490 | |
| <I/σ(I)> | 11.3 | 11.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 40 | 41.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 0.1M NaCl, 0.1M HEPES pH 7.5, 1.6M Ammonium Sulphate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
