3KHK

Crystal structure of type-I restriction-modification system methylation subunit (MM_0429) from Methanosarchina mazei.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 41
Unit cell lengths	80.648, 80.648, 179.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.550
R-factor	0.214
Rwork	0.213
R-free	0.24200
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.355
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.590
High resolution limit [Å]	2.500	2.550
Rmerge	0.098	0.816
Number of reflections	37205
<I/σ(I)>	25.8	3
Completeness [%]	100.0	100
Redundancy	10.6	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	Sodium acetate trihydrate pH 4.6, 25% PEG 3350,0.2M Ammonium Acetate,0.05M Magnesium Sulfate , VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	Sodium acetate trihydrate pH 4.6, 25% PEG 3350,0.2M Ammonium Acetate,0.05M Magnesium Sulfate , VAPOR DIFFUSION, SITTING DROP, temperature 298K