3KH9
Crystal structure of the periplasmic soluble domain of oxidized CcmG from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-08 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.91841 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 74.099, 74.099, 65.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.140 - 2.200 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.23000 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.184 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.414 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.077 | 0.300 |
Total number of observations | 9974 | |
Number of reflections | 9665 | |
<I/σ(I)> | 22.2 | 2.1 |
Completeness [%] | 99.5 | 97.2 |
Redundancy | 7.6 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 18-20% PEG 4000, 0.1-0.3M Magnesium formate, 0.1M sodium citrate pH 5.6, 5% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 294K |