3KH8
Crystal structure of MaoC-like dehydratase from Phytophthora Capsici
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.458, 82.614, 124.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.490 - 2.000 |
| R-factor | 0.20006 |
| Rwork | 0.198 |
| R-free | 0.22977 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s9c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.490 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.111 | 0.715 |
| Number of reflections | 62446 | |
| <I/σ(I)> | 19.6 | 2.56 |
| Completeness [%] | 97.9 | 95.5 |
| Redundancy | 6.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M Hepes, 6% PEG6000, 5% (+/-)-2-methy-2,4-pentanediol , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
