3KH7
Crystal structure of the periplasmic soluble domain of reduced CcmG from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 74.345, 74.345, 66.746 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kng |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.362 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.090 | 0.394 |
| Number of reflections | 19387 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14.3 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 18-20% PEG 4000 W/V, 0.1-0.3 M magnesium formate, 0.1M sodium citrate pH 5.6, 5% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
