3KH3
Crystal structure of human Cu/Zn superoxide dismutase recombinantly produced in Leishmania tarantolae; P212121 crystal form containing 12 chains in the asymmetric unit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.500, 166.610, 174.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.780 - 3.500 |
R-factor | 0.244 |
Rwork | 0.243 |
R-free | 0.27700 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.173 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.600 | |
High resolution limit [Å] | 3.500 | 50.000 | 3.500 |
Rmerge | 0.189 | 0.042 | 0.575 |
Number of reflections | 28607 | 9 | 2306 |
<I/σ(I)> | 11.16 | 28.9 | 4.3 |
Completeness [%] | 99.9 | 60 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 21-25% (w/v) PEG4000, 0.1 M NaOAc, pH 4.2-5.2, vapor diffusion, hanging drop, temperature 292K |