3KH0

Crystal structure of the Ras-association (RA) domain of RALGDS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97920
Spacegroup name	P 61
Unit cell lengths	104.590, 104.590, 39.819
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.680 - 2.100
R-factor	0.205
Rwork	0.204
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lxd
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.161	0.089	0.658
Number of reflections	14288
<I/σ(I)>	5.8
Completeness [%]	96.7	99.8	82.5
Redundancy	6.7	10.8	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	2.0M ammonium sulfate, 2.0% PEG400, 0.1m sodium HEPES. 1:100 (w/w) endoproteinase Glu-C were present in the crystallization drop, pH 7.5, vapor diffusion, sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP