3KGX
Crystal structure of Putative aminotransferase (AAH25799.1) from MUS MUSCULUS at 1.80 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-18 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.391, 112.565, 117.351 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.696 - 1.800 |
R-factor | 0.175 |
Rwork | 0.173 |
R-free | 0.20700 |
Structure solution method | MR |
Starting model (for MR) | 3kgw |
RMSD bond length | 0.015 |
RMSD bond angle | 1.832 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.696 | 29.700 | 1.850 |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.082 | 0.035 | 0.684 |
Total number of observations | 3162 | 20105 | |
Number of reflections | 76087 | ||
<I/σ(I)> | 10.7 | 15.2 | 1.1 |
Completeness [%] | 99.9 | 97.8 | 100 |
Redundancy | 3.5 | 3.3 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 20.0000% polyethylene glycol 3350, 0.2000M ammonium fluoride, No Buffer pH 6.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |