3KGS
V30M mutant human transthyretin (TTR) (apoV30M) pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.4586 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.388, 85.463, 64.233 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.100 - 1.800 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f41 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.375 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.063 | 0.063 |
Number of reflections | 26041 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 97.0 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M CaCl2, 0.1 M HEPES pH 7.5, 28% PEG 400, vapor diffusion, hanging drop, temperature 293K |