3KGD
Crystal structure of E. coli RNA 3' cyclase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2009-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.10000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.728, 81.932, 105.176 |
| Unit cell angles | 90.00, 103.44, 90.00 |
Refinement procedure
| Resolution | 35.137 - 1.680 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qmh |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.461 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.4_125) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.700 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.074 | 0.551 |
| Number of reflections | 155565 | |
| <I/σ(I)> | 10.5 | 2.13 |
| Completeness [%] | 93.9 | 96.7 |
| Redundancy | 6.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 100 mM Na MES pH 6.5, 200 mM Ammonium sulfate, 20% w/v PEG 5000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
