3KG4
Crystal structure of an uncharacterized protein from Mannheimia succiniciproducens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97958 |
Spacegroup name | P 63 |
Unit cell lengths | 81.827, 81.827, 61.937 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.24200 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.501 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.864 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.086 | 0.330 |
Number of reflections | 17142 | |
<I/σ(I)> | 25.7 | 10.2 |
Completeness [%] | 99.0 | 98.4 |
Redundancy | 23.1 | 23.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 294 | 100mM Bis-Tris pH 5.5, 22% PEG 3350, 200mM ammonium sulfate, VAPOR DIFFUSION, temperature 294K |