3KFI

Major mouse urinary protein IV complexed with 2,5-dimethylpyrazine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-10-06
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	47.838, 53.444, 61.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.800 - 1.420
R-factor	0.15
Rwork	0.148
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MUP-I (PDB ENTRY 1I06)
RMSD bond length	0.015
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.470
High resolution limit [Å]	1.420	1.420
Rmerge	0.054	0.277
Number of reflections	29102
<I/σ(I)>	16.3	2.4
Completeness [%]	95.1	83.2
Redundancy	2.9	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	298	0.2-0.5 M calcium chloride dihydrate, 20% (w/v) polyethylene glycol 3350, pH 6, vapor diffusion, temperature 298K