3KF9

Crystal structure of the SdCen/skMLCK complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	200
Detector technology	CCD
Collection date	2009-09-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9756
Spacegroup name	P 21 21 21
Unit cell lengths	40.750, 67.750, 129.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.600
R-factor	0.20427
Rwork	0.200
R-free	0.29021
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qtx
RMSD bond length	0.014
RMSD bond angle	1.451
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.104	0.235
Number of reflections	10844
<I/σ(I)>	10.1	6.3
Completeness [%]		95.3
Redundancy	3.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	MPEG 2000, Nacacodylate 0.1M, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K