3KF3
Structure of fructofuranosidase from Schwanniomyces occidentalis complexed with fructose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.870, 92.270, 116.280 |
| Unit cell angles | 90.00, 104.81, 90.00 |
Refinement procedure
| Resolution | 49.620 - 1.900 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kf5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.425 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4_3.3.9) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.420 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.126 | 0.450 |
| Number of reflections | 114733 | |
| <I/σ(I)> | 4.3 | 1.9 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% PEG 6000, 0.2M Cl2Mg, 0.1M Hepes, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
