3KEU
Crystal Structure of Human PL Kinase with bound PLP and ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-08-28 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.54 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 91.202, 114.585, 169.414 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.980 - 2.100 |
Rwork | 0.209 |
R-free | 0.25600 |
Structure solution method | isomorphous refinement |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.2SSI) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 26.980 | 26.980 | 2.070 |
High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
Rmerge | 0.049 | 0.019 | 0.300 |
Total number of observations | 22160 | 18558 | |
Number of reflections | 59477 | ||
<I/σ(I)> | 13.3 | 44 | 3.4 |
Completeness [%] | 98.9 | 96.5 | 99 |
Redundancy | 3.1 | 3.37 | 2.81 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 8 | 298 | Saturated MgATP and PLP, 50-60% MPD precipitant, pH 8.0, hanging drop, temperature 298K |