3KET
Crystal structure of a Rex-family transcriptional regulatory protein from Streptococcus agalactiae bound to a palindromic operator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0379 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 48.720, 107.030, 153.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.490 - 2.400 |
| R-factor | 0.19757 |
| Rwork | 0.195 |
| R-free | 0.24718 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3KEO and a fiber model double stranded B-DNA of length 11 base pairs |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.203 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.490 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.106 | 0.460 |
| Number of reflections | 16157 | |
| <I/σ(I)> | 10.2 | 3.04 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 4.67 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 22 % PEG 1500, 50mM MIB, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
