3KEI
Crystal Structure of the GluA4 Ligand-Binding domain L651V mutant in complex with glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.200, 105.100, 66.300 |
| Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
| Resolution | 19.690 - 1.900 |
| Rwork | 0.205 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3epe |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.091 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 45551 | |
| Completeness [%] | 90.2 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 24% PEG 4000, 0.1M Na-Acetate, 0.3M Ammonium Sulphate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
