3KEH
Crystal Structure of N370S Glucocerebrosidase mutant at pH 7.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.830, 285.920, 92.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.21473 |
| Rwork | 0.212 |
| R-free | 0.27347 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | !OGS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.605 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.000 | 2.880 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 31773 | |
| <I/σ(I)> | 8.4 | 2.3 |
| Completeness [%] | 94.7 | 91.1 |
| Redundancy | 5.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 294 | 0.7M Potassium Phosphate/0.7M Sodium Phosphate/0.1M HEPES, pH7.4, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
