3KE3
Crystal structure of Putative serine-pyruvate aminotransferase (YP_263484.1) from PSYCHROBACTER ARCTICUM 273-4 at 2.20 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-21 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91162,0.97956,0.97938 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.645, 98.352, 121.072 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.867 - 2.200 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.21000 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.543 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.867 | 29.870 | 2.260 |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.125 | 0.061 | 0.652 |
Total number of observations | 740 | 5143 | |
Number of reflections | 19035 | ||
<I/σ(I)> | 8.8 | 9.7 | 1.2 |
Completeness [%] | 99.8 | 95 | 99.9 |
Redundancy | 3.7 | 3.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 25.0000% polyethylene glycol 4000, 0.2000M ammonium sulfate, 0.1M sodium acetate pH 4.6, Additive: 0.001M pyridoxal-5'-phospate (PLP), NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 293K |