3KDR
The Crystal Structure of a HK97 Family Phage Portal Protein from Corynebacterium diphtheriae to 2.9A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 340.068, 340.068, 340.068 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.250 - 2.900 |
R-factor | 0.19993 |
Rwork | 0.198 |
R-free | 0.24503 |
RMSD bond length | 0.022 |
RMSD bond angle | 1.776 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (refmac_5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.950 |
High resolution limit [Å] | 2.900 | 7.850 | 2.900 |
Rmerge | 0.149 | 0.059 | 0.742 |
Number of reflections | 37241 | ||
<I/σ(I)> | 5.6 | ||
Completeness [%] | 98.5 | 94.2 | 99.7 |
Redundancy | 9 | 9.2 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 277 | 1.2M Sodium phosphate dibasic/0.8M Potassium phosphate monobasic, CAPS pH 10.5, 0.2M Lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |