3KDK
Structure of the C-terminal domain of Bacillus subtilis MutL bound to Zn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.231, 74.608, 182.244 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.750 - 2.260 |
R-factor | 0.2169 |
Rwork | 0.214 |
R-free | 0.26850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gab |
RMSD bond length | 0.004 |
RMSD bond angle | 0.745 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.083 | 0.453 |
Number of reflections | 41093 | |
<I/σ(I)> | 20.8 | 4.2 |
Completeness [%] | 99.7 | 97.4 |
Redundancy | 6.5 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 277 | 25% PEG 3350, 0.2 M NaCl, 0.1 mM ZnCl2, 0.1 M TRIS pH 7.0, VAPOR DIFFUSION, temperature 277K |