3KD8
Cofactor-Independent Phosphoglycerate mutase from Thermoplasma Acidophilum DSM 1728
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97880, 0.97904 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.911, 137.081, 67.088 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.920 - 2.600 |
| R-factor | 0.21145 |
| Rwork | 0.207 |
| R-free | 0.28782 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.643 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.920 | 2.620 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.105 | 0.509 |
| Number of reflections | 25760 | |
| <I/σ(I)> | 30.75 | 2.923 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 9.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 296 | 0.16M magnesium chloride 0.08M tris HCl, pH 8.5 24% PEG 4000 20% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
