3KCV
Structure of formate channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 107.034, 107.034, 285.452 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.610 - 3.198 |
| R-factor | 0.1967 |
| Rwork | 0.193 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kcu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.607 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.610 | 3.310 |
| High resolution limit [Å] | 3.198 | 3.198 |
| Rmerge | 0.100 | 0.729 |
| Number of reflections | 60335 | |
| <I/σ(I)> | 13.3 | 1.6 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M MOPS, pH 7.5, 36% (w/v) PEG 400, 200mM NaCl or sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
