3KCC
Crystal structure of D138L mutant of Catabolite Gene Activator Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-26 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.536, 102.222, 53.912 |
| Unit cell angles | 90.00, 110.93, 90.00 |
Refinement procedure
| Resolution | 39.268 - 1.660 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hw5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.218 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.058 | 0.483 |
| Number of reflections | 55401 | |
| <I/σ(I)> | 27.8 | 3.8 |
| Completeness [%] | 89.5 | 96.4 |
| Redundancy | 7.3 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 10% PEG4000, 7% isopropanol, 3mM cAMP, 0.1M HEPES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
