3KC2
Crystal structure of mitochondrial HAD-like phosphatase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-04 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.734, 67.095, 76.899 |
Unit cell angles | 90.00, 110.45, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.550 |
R-factor | 0.145 |
Rwork | 0.144 |
R-free | 0.16900 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.509 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.063 | 0.766 |
Number of reflections | 99129 | |
<I/σ(I)> | 24.3 | 1.9 |
Completeness [%] | 99.6 | 98.9 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 293 | 200mM Na,K phosphate, Hepes, 2mM MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |