3KBL

Crystal structure of the GLD-1 homodimerization domain from Caenorhabditis elegans N169A mutant at 2.28 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Detector technology	CCD
Collection date	2008-12-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	34.406, 42.300, 156.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.192 - 2.280
R-factor	0.199
Rwork	0.195
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CS-Rosetta model of wildtype construct with sidechain of N169 changed to A to account for mutation
RMSD bond length	0.008
RMSD bond angle	1.011
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.280	2.280
Rmerge	0.066	0.283
Number of reflections	11001
<I/σ(I)>	12.1	4.37
Completeness [%]	99.4	94.1
Redundancy	7.4	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	MES-Na, PEG 200, PEG 3000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K