3KB5

PRY-SPRY domain of human TRIM72

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97951, 0.97967, 0.9600
Spacegroup name	C 1 2 1
Unit cell lengths	153.747, 35.937, 33.200
Unit cell angles	90.00, 99.87, 90.00

Refinement procedure

Resolution	29.280 - 1.500
Rwork	0.219
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.313
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	26011
Completeness [%]		99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1M Tris-HCl, 20% (w/v) polyethylene glycol 3350, 0.2M potassium nitrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1M Tris-HCl, 20% (w/v) polyethylene glycol 3350, 0.2M potassium nitrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K