3KB1
Crystal Structure of the Nucleotide-binding protein AF_226 in complex with ADP from Archaeoglobus fulgidus, Northeast Structural Genomics Consortium Target GR157
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97899 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.440, 67.682, 79.403 |
Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.26500 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELX (then SOLVE/RESOLVE) |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.093 | 0.254 |
Number of reflections | 26385 | |
<I/σ(I)> | 15 | 3.07 |
Completeness [%] | 98.3 | 88.1 |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 100 mM NaAcetate (pH 4.6), 30% MPD, and 200 mM NaCl. , VAPOR DIFFUSION, SITTING DROP, temperature 291K |