3KAR
THE MOTOR DOMAIN OF KINESIN-LIKE PROTEIN KAR3, A SACCHAROMYCES CEREVISIAE KINESIN-RELATED PROTEIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 269 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-04-09 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.129, 81.209, 48.298 |
| Unit cell angles | 90.00, 105.78, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| Rwork | 0.175 |
| Structure solution method | MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 19.650 * |
| Data reduction software | XDS |
| Data scaling software | XSCALIBRE |
| Phasing software | HEAVY |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.034 | 0.074 |
| Total number of observations | 43898 * | |
| Number of reflections | 22220 | |
| Completeness [%] | 93.0 | 84.7 * |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 | 11 % MEPEG 2000, 50 MM NACL, 1% ETHYLENE GLYCOL, 1 MM MG ADP, pH 7. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 2 (mM) | |
| 2 | 1 | 1 | methyl ether PEG2000 | 22 (%) | |
| 3 | 1 | 1 | 100 (mM) | ||
| 4 | 1 | 1 | ethylene glycol | 2 (%) | |
| 5 | 1 | 1 | HEPES | 50 (mM) |
