3KAP
Flavodoxin from Desulfovibrio desulfuricans ATCC 27774 (oxidized form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-02-22 |
| Detector | MAR scanner 180 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.900, 95.900, 33.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.050 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fx1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.934 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.140 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.082 | 0.320 |
| Number of reflections | 10938 | |
| Completeness [%] | 94.2 | 93.8 |
| Redundancy | 4.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | Crystals were obtained at room temperature from a protein solution of 8-10mg/ml in a solution containing 10% MPD, 2.3M ammonium sulfate, 0.1 M sodium acetate (pH 5.0), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
