3KA3
Frog M-ferritin with magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1159 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 183.886, 183.886, 183.886 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.400 |
R-factor | 0.137 |
Rwork | 0.136 |
R-free | 0.15900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.028 |
RMSD bond angle | 2.141 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.480 |
High resolution limit [Å] | 1.400 | 4.430 | 1.400 |
Rmerge | 0.088 | 0.038 | 0.969 |
Total number of observations | 23618 | 103581 | |
Number of reflections | 52741 | ||
<I/σ(I)> | 21.2 | 10.7 | 0.8 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 13.6 | 12.4 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 2M MgCl2, 0.1M Bicine pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |