3K9N
Allosteric modulation of H-Ras GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 38.423, 38.423, 191.749 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.335 - 2.000 |
| R-factor | 0.2198 |
| Rwork | 0.213 |
| R-free | 0.27680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rge |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.144 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.143 | 0.397 |
| Number of reflections | 10450 | |
| <I/σ(I)> | 12.45 | 1.16 |
| Completeness [%] | 87.5 | 86.1 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.5 | 0.2M Calcium Acetate, 20 % PEG 3350, 5 mM MgCl2, 10 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.5K |
