3K9L
Allosteric modulation of H-Ras GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.113, 60.632, 74.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.316 - 1.800 |
| R-factor | 0.2207 |
| Rwork | 0.219 |
| R-free | 0.27080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rge |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.152 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.900 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.100 | 0.840 |
| Number of reflections | 48368 | |
| <I/σ(I)> | 13.1 | 1.45 |
| Completeness [%] | 96.0 | 89.6 |
| Redundancy | 4.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.2M Calcium Acetate, 5 mM MgCl2, 10 mM DTT, 20% PEG 3350., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
