3K9K
Transposase domain of Metnase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.107203 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.990, 82.990, 289.779 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.872 - 2.550 |
| R-factor | 0.234 |
| Rwork | 0.231 |
| R-free | 0.29080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.028 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.4_4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.058 | 0.727 |
| Number of reflections | 20325 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 11.1 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | NH4OAc, CaCl2, PEG4000, pH 8.0, vapor diffusion, temperature 293K |
