3K9H
Crystal structure of a plasmid partition protein from borrelia burgdorferi at 2.25A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-29 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 |
Unit cell lengths | 57.000, 57.000, 343.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.200 - 2.250 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k9g iodide soak |
RMSD bond length | 0.017 |
RMSD bond angle | 1.497 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.200 | 2.310 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.072 | 0.326 |
Number of reflections | 28812 | |
<I/σ(I)> | 18.08 | 3.7 |
Completeness [%] | 96.4 | 84 |
Redundancy | 6.3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | INDEX SCREEN G5: 25% PEG 3350, TRIS PH 8.5, 200MM LI2SO4, BOBUA.01478.A AT 33MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |