3K93
Crystal structure of phage related exonuclease (YP_719632.1) from HAEMOPHILUS SOMNUS 129PT at 2.15 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-08 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97947 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 113.369, 113.369, 42.284 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.341 - 2.150 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.21200 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.459 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.341 | 28.340 | 2.210 |
High resolution limit [Å] | 2.150 | 9.620 | 2.150 |
Rmerge | 0.129 | 0.055 | 0.836 |
Total number of observations | 1365 | 9647 | |
Number of reflections | 17252 | ||
<I/σ(I)> | 12.8 | 11.5 | 2.1 |
Completeness [%] | 100.0 | 96.6 | 100 |
Redundancy | 7.4 | 6.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 10.0000% Glycerol, 1.0000M NaCl, 30.0000% PEG-600, 0.1M Cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |