3K8Z
Crystal Structure of Gudb1 a decryptified secondary glutamate dehydrogenase from B. subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.220, 192.491, 89.387 |
| Unit cell angles | 90.00, 118.74, 90.00 |
Refinement procedure
| Resolution | 19.769 - 2.400 |
| R-factor | 0.249 |
| Rwork | 0.247 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.943 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 96102 | |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 20% Peg 3350, 0.15M sodium malonate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
