3K8W
Crysatl structure of a bacterial cell-surface flagellin N20C45
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-09 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.894, 71.317, 50.976 |
| Unit cell angles | 90.00, 117.13, 90.00 |
Refinement procedure
| Resolution | 37.690 - 1.700 |
| R-factor | 0.19796 |
| Rwork | 0.197 |
| R-free | 0.22259 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zbi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.825 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.232 |
| Number of reflections | 34987 | |
| Completeness [%] | 99.8 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
