3K8R

Crystal structure of protein of unknown function (YP_427503.1) from Rhodospirillum rubrum ATCC 11170 at 2.75 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97932
Spacegroup name	I 4
Unit cell lengths	111.970, 111.970, 45.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.855 - 2.750
R-factor	0.214
Rwork	0.213
R-free	0.23500
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.492
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.855	28.855	2.850
High resolution limit [Å]	2.750	5.880	2.750
Rmerge	0.076	0.034	0.616
Number of reflections	7459	1442	1391
<I/σ(I)>	8.8	19.2	1.5
Completeness [%]	97.9	97.6	94.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	20.0000% PEG-6000, 0.1M TRIS pH 8.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K