3K8K
Crystal structure of SusG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-30 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 41 |
| Unit cell lengths | 128.038, 128.038, 129.774 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| Rwork | 0.196 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.217 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 94667 | |
| <I/σ(I)> | 28.1 | 3 |
| Completeness [%] | 91.0 | |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 11mg/ml protein, 100mM HEPES, 200mM Magnesium Acetate, 18% PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
