3K8C
Complex of Trypanosoma cruzi ribose 5-phosphate isomerase type B with 4-deoxy-4-phospho-D-erythronohydroxamic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 92.387, 92.387, 94.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.21859 |
| Rwork | 0.217 |
| R-free | 0.25336 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k7p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.396 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.938 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.097 | 0.451 |
| Number of reflections | 23175 | |
| <I/σ(I)> | 14.7 | 1.3 |
| Completeness [%] | 96.0 | 95.9 |
| Redundancy | 4.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% (w/v) PEG 6000, 0.2 M ammonium chloride, 0.1 M Tris-HCl, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
